- Resource Types
- Resource Languages
- Institutional Repository
Keep Browsing
WHDL - 00020147
Visit the home page
About Site Language
WHDL is viewable in multiple languages. Use the pull-down menu to select a language to view the site.
I changed my language, but I’m still seeing resources in the other languages?
If a resource or text has not been translated into your selected language, it will appear in the initially added language. We are always looking for help translating these resources. If you can help, contact us!
Cover Image
Description / Abstract
Bacteriophage lambda procapsids have shown promising uses as viral nanoparticles, as its structure allows for the possibility of drug encapsulation. The icosahedral procapsid consists of several individual proteins: the major capsid protein - gpE, head decoration protein - gpD, scaffolding protein - gpNu3, portal protein - gpB, and protease - gpC. In previous research, the in vitro assembly of the procapsids was attempted using only gpE, gpD, and gpNu3, with success in aggregation but not uniformity. The purpose of this project is to determine the effect of the presence of gpB and gpC on assembly, by cloning these proteins to have a glutathione-S-transferase (GST) affinity tag. After purification and incorporation of gpB and gpC, uniformity of procapsid formation improves.
Download this Resource
Download Free from WHDL
-
Download as a PDF: Honors Paper
File Size Small - 2.7 MB
- Publish Year
KeywordsBacteriophage Lambda, Procapsid Assembly, Viral Nanoparticles, Targeted Drug Delivery, Cloning, GST-affinity chromatography
Degree AwardedB.S. in Biology-Chemistry & B.A. in Spanish (Bethany Arabe), B.S. in Biology (Madelyn Stueve), B.S. in Biology (Tobias Wakefield-Carl)
Awarding InstitutionPoint Loma Nazarene University
Committee MembersKristopher Koudelka, Ariane Jansma, Michael Dorrell
Periodical TitleArete: The PLNU Honors Journal
Peer Reviewed?No
Collections With This Resource
PLNU Undergraduate Honors Projects 2021-2025
69 Resources
- English
Related Resources
Predator-prey relationships for coyote (Canis latrans), ocelot (Leopardus pardalis), and puma (Puma concolor) in Costa Rican ecosystems
- Academic (Thesis, Dissertation, Etc.)
- English
A comparative study between two approaches to university STEM education in the Democratic Republic of Congo: The effects of inquiry-learning on student learning outcomes and plagiarism rates
- Academic (Thesis, Dissertation, Etc.)
- English
2022
A Revision of the Conceptual Inventory of Natural Selection for Middle School Students
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of Genes Expressed in Immunoglobulin M B Cells
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of Pencil Urchin Population Genetics at Hannibal Bank
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of the Diversity and Community Structure of Fauna at Hannibal Bank Based on Imagery Data
- Academic (Thesis, Dissertation, Etc.)
- English
Addressing Quantitative Literacy Gaps: Use of an Intervention to meet the needs of Struggling STEM Majors
- Academic (Thesis, Dissertation, Etc.)
- English
2024
An Anatomical and Physiological Study on the Effects of Whiplash
- Academic (Thesis, Dissertation, Etc.)
- English
An Exploration of Graphing Software Program Selection and Relative Proficiency Among Undergraduate Biology Students
- Academic (Thesis, Dissertation, Etc.)
- English
Analysis of Plasmids Expressing Bacteriophage Lambda Procapsid
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Behavior Enrichments for the Stress of Wolves
- Media
- English
Bioassay-guided Isolation of a Natural Product Inhibitor of Epicoccum Nigrum to Combat Citrus Greening Disease
- Academic (Thesis, Dissertation, Etc.)
- English
Cancer Proliferation Mediated by Tumor-Associated Macrophages (TAMs)
- Academic (Thesis, Dissertation, Etc.)
- English
Cloning and Expression of Putative Antibiotic Resistance Gene, eamA, from Environmental Plasmid pTRE-2011
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Comparison of Massed Versus Distributed Use of Natural Selection Concept Cartoons in a Secondary Biology Curriculum
- Academic (Thesis, Dissertation, Etc.)
- English
Comprehensive Single-Season Occupancy Analysis with Covariates for all Species within Genus Tapirus
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Conducting a Time Course of Bacteria Strain to Investigate the Growing Conditions of CB729, a Citrus Native Bacteria
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Creationist-Believing Students’ Scientific Knowledge and Reasoning about Evolution
- Academic (Thesis, Dissertation, Etc.)
- English
Creativity and Precision in Protein Folding
- Media
- English
CRISPR/Cas9 Mediated Mutagenesis of the ELOVL4 Gene to Generate a Patient Specific Stargardt’s Eye Disease Model
- Academic (Thesis, Dissertation, Etc.)
- English
2023
CXCL14 as Potential Binding Partner for HPV E7
- Academic (Thesis, Dissertation, Etc.)
- English
2024
Dairy Farming: An Assessment of the Sustainability of Dairy Farms Across the United States and in Bond County
- Academic (Thesis, Dissertation, Etc.)
- Unpublished Text
- English
2020
Degradation of pRb by Retrovirally Infected Cell Lines
- Academic (Thesis, Dissertation, Etc.)
- English
Development and field testing of a concept cartoon intervention to improve students’ understandings of the role of genetics in natural selection as a mechanism of evolution
- Academic (Thesis, Dissertation, Etc.)
- English
2021
Elucidating Population Genetics of Shrimp Species On Underwater Volcanoes
- Academic (Thesis, Dissertation, Etc.)
- English
2024